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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2979-0.5 | 0.5 mg (10 mg/ml) | $440 |
IHC‑plus™ Polyclonal Sheep anti‑Human F8 / FVIII / Factor VIII Antibody (IHC) LS‑B2979
IHC‑plus™ Polyclonal Sheep anti‑Human F8 / FVIII / Factor VIII Antibody (IHC) LS‑B2979
Note: This antibody replaces LS-C23379
Antibody:
F8 / FVIII / Factor VIII Sheep anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
F8 / FVIII / Factor VIII Sheep anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Factor VIII antibody LS-B2979 is an unconjugated sheep polyclonal antibody to human Factor VIII (F8 / FVIII). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human F8 / FVIII / Factor VIII
Synonyms
F8 | AHF | Antihemophilic factor | Coagulation factor VIIIc | Coagulation factor VIII | DXS1253E | Factor VIII F8B | FVIII | HEMA | F8B | Factor VIII | Procoagulant component | F8C
Host
Sheep
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Caprylic acid and ammonium sulfate precipitations and solid-phase adsorption
Modifications
Unmodified
Immunogen
Human Factor VIIIC (F. VIII) purified from F. VIII concentrate.
Specificity
Recognizes human F. VIII as demonstrated by immunodiffusion and ELISA. No reactivity was observed with von Willebrand Factor.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2979 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2979 was determined to be 5 ug/ml.
Presentation
10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About F8 / FVIII / Factor VIII
Validation
Anti-F8 / Factor VIII antibody IHC of human skeletal muscle and capillaries. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-F8 / Factor VIII antibody IHC of human skeletal muscle and capillaries. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ F8 / FVIII / Factor VIII Antibody for IHC, ELISA LS-B2979 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
IL-15 blockade and rapamycin rescue multifactorial loss of factor VIII from AAV-transduced hepatocytes in hemophilia A mice. John S S Butterfield, Kentaro Yamada, Thais B Bertolini, Farooq Syed, Sandeep R P Kumar, Xin Li, Sreevani Arisa, Annie R Piñeros, Alejandro Tapia, Christopher A Rogers, Ning Li, Jyoti Rana, Moanaro Biswas, Cox Terhorst, Randal J Kaufman, Ype P de Jong, Roland W Herzog. Molecular therapy : the journal of the American Society of Gene Therapy. 2022 December;30:3552-3569.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Western blot
Species:
Human
Applications:
ELISA, Immunoelectrophoresis
Species:
Pig
Applications:
Western blot
Reactivity:
Human
Range:
0.78-50 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
