Target
Human ERCC1
Synonyms
ERCC1 | COFS4 | UV20 | RAD10
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence DPGKDKEGVPQPS-C, from the N-Terminus of protein sequence according to NP_973730.1NP_001974.1.
Specificity
Human ERCC1. This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38 kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6 kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee