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Catalog Number	Size	Price
LS-B3956-50	50 µg (0.5 mg/ml)	$485

ERCC1 Antibody - Anti-ERCC1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

ERCC1 Antibody - Goat Anti-ERCC1 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

ERCC1 Antibody - Goat Anti-ERCC1 Antibody Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

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IHC‑plus™ Polyclonal Goat anti‑Human ERCC1 Antibody (aa172‑184, IHC, IF) LS‑B3956

Name: IHC-plus™ ERCC1 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B3956-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C109075

Antibody:

ERCC1 Goat anti-Human Polyclonal (aa172-184) Antibody

Application:

IHC, IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog

Format:

Unconjugated, Unmodified

$485

LS-B3956-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ERCC1 Goat anti-Human Polyclonal (aa172-184) Antibody

Application:

IHC, IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

ERCC1 antibody LS-B3956 is an unconjugated goat polyclonal antibody to ERCC1 (aa172-184) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.

Target

Human ERCC1

Synonyms

ERCC1 | COFS4 | UV20 | RAD10

See All ERCC1 Antibodies

Host

Goat

Reactivity

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-QVDVKDPQQALKE, from the internal region of the protein sequence according to NP_973730.1NP_001974.1NP_001159521.1.

Epitope

aa172-184

Specificity

Human ERCC1.

Applications

IHC
IHC - Paraffin (3.75 µg/ml)
Immunofluorescence (10 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B3956 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3956 was determined to be 3.75 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ERCC1

Uniprot: P07992 NCBI: NP_973730.1

Validation

Anti-ERCC1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

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About LSBio Validation process