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Antibodies > ERBB3 / HER3 > LS-C356018
 
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Catalog Number Size Price
LS-C356018-100 100 µl (1 mg/ml) $315 
ERBB3 / HER3 Antibody - Immunohistochemical analysis of HER3 (pY1328) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ERBB3 / HER3 Antibody - Immunofluorescent analysis of HER3 (pY1328) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ERBB3 / HER3 Antibody - Western blot analysis of HER3 (pY1328) expression in A431 EGF-treated (A); Raw264.7 (B) whole cell lysates.
ERBB3 / HER3 Antibody - Immunohistochemical analysis of HER3 (pY1328) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ERBB3 / HER3 Antibody - Immunofluorescent analysis of HER3 (pY1328) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ERBB3 / HER3 Antibody - Western blot analysis of HER3 (pY1328) expression in A431 EGF-treated (A); Raw264.7 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human ERBB3 / HER3 Antibody (C‑Terminus, IHC, IF, WB) LS‑C356018

Polyclonal Rabbit anti‑Human ERBB3 / HER3 Antibody (C‑Terminus, IHC, IF, WB) LS‑C356018

Submit Review LSBio Guarantee
Antibody:
ERBB3 / HER3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C356018-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ERBB3 / HER3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
HER3 antibody LS-C356018 is an unconjugated rabbit polyclonal antibody to HER3 (ERBB3) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human ERBB3 / HER3
Synonyms
ERBB3 | C-erbB-3 | C-erbB3 | ErbB-3 | HER3 | p85-sErbB3 | p45-sErbB3 | MDA-BF-1 | ErbB3-S | LCCS2 | p180-ErbB3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human HER3 (pY1328).
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of HER3 (pY1328) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ERBB3 / HER3
P21860 NM_001982 NP_001973.2

Publications (0)

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Featured Products

ERBB3 / HER3 Antibody - Anti-HER3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ ERBB3 / HER3 Antibody (clone 3F10F6) LS‑B3896
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
ERBB3 / HER3 Antibody - Anti-HER3 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PathPlus™ ERBB3 / HER3 Antibody (aa1300‑C‑term) LS‑B2126
Species: Human, Monkey, Rat, Bat, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
ERBB3 / HER3 Antibody - Anti-HER3 antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ ERBB3 / HER3 Antibody (clone 2E9) LS‑B6035
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
ERBB3 / HER3 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
ERBB3 / HER3 Antibody (aa24‑55) LS‑C97504
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Product
ERBB3 / HER3 Antibody (phospho‑Tyr1222) LS‑C35220
Species: Human
Applications: Western blot, ELISA
ERBB3 / HER3 Antibody - Immunohistochemical analysis of HER3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ERBB3 / HER3 Antibody (N‑Terminus) LS‑C368595
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all ERBB3 / HER3 Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/30/2024
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