Human EPHA3 / EPH Receptor A3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide derived from the C-Terminus of the human EphA3 receptor.

C-Terminus

On Western Blot analysis, this antibody reacts specifically with recombinant human EphA3 receptor without cross-reaction with EphA4 receptor expressed in transfected HEK-293 cells. The antibody reactivity was confirmed by Western Blot and Immunoprecipitation analyses. Reactivity has been confirmed with human EphA3 receptor transfected HEK-293 cell lysate. The molecular weight for human EphA3 receptor is ~110kD on Western blots. Based on sequence homology, the antibody should also recognize the rat or mouse EphA3 receptor.

• Western blot (1 - 3 µg/ml)
• Immunoprecipitation
• ELISA (0.1 - 1 µg/ml)

Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml, Band at ~110kD. Immunoprecipitation: 5-10 ug. Positive control: Human EphA3 receptor transfected HEK-293 cell lysate.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P29320 NCBI: NM_005233 NP_005224.2