Human EPCAM

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

VU-1D9

Unconjugated

Affinity purified

Low Endotoxin

HG9 cell line (small cell lung carcinoma).

Recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kD cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot
• Immunoprecipitation
• Flow Cytometry (1:10 - 1:200)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product requires protein digestion pre-treatment of paraffin sections e. g. trypsin. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16422 NCBI: NM_002354 NP_002345.2