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Monoclonal Mouse anti‑Human ENO2 / NSE Antibody (clone ENO2/1462, Azide‑free,BSA‑free, IHC, IF) LS‑C761796
Monoclonal Mouse anti‑Human ENO2 / NSE Antibody (clone ENO2/1462, Azide‑free,BSA‑free, IHC, IF) LS‑C761796
Antibody:
ENO2 / NSE Mouse anti-Human Monoclonal (Azide-free, BSA-free) (ENO2/1462) Antibody
Application:
IHC-P, IF, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
ENO2 / NSE Mouse anti-Human Monoclonal (Azide-free, BSA-free) (ENO2/1462) Antibody
Application:
IHC-P, IF, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
NSE antibody LS-C761796 is an unconjugated mouse monoclonal antibody to human NSE (ENO2). Validated for Flow, IF and IHC.
Target
Human ENO2 / NSE
Synonyms
ENO2 | Enolase 2 | Enolase 2 (gamma, neuronal) | Neurone-specific enolase | Gamma-enolase | Neural enolase | Neuron specific gamma enolase | Neuron-specific enolase | NSE
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free.
Also available
Unmodified or
Ready-to-Use.
Immunogen
Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
Applications
- IHC - Paraffin (0.1 - 0.2 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Prediluted IHC only format: incubate for 30 min at RT
Storage
Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human, Monkey, Mouse, Rat, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 6/29/2024