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Catalog Number Size Price
LS-C331216-20 20 µl $287 
LS-C331216-100 100 µl $431 
ENO1 / Alpha Enolase Antibody - Immunofluorescence analysis of HeLa cells using ENO1 antibody. Blue: DAPI for nuclear staining.
ENO1 / Alpha Enolase Antibody - Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibodyat 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
ENO1 / Alpha Enolase Antibody - Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
ENO1 / Alpha Enolase Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.
ENO1 / Alpha Enolase Antibody - Immunofluorescence analysis of HeLa cells using ENO1 antibody. Blue: DAPI for nuclear staining.
ENO1 / Alpha Enolase Antibody - Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibodyat 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
ENO1 / Alpha Enolase Antibody - Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
ENO1 / Alpha Enolase Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.
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Polyclonal Rabbit anti‑Human ENO1 / Alpha Enolase Antibody (IHC, IF, WB) LS‑C331216

Polyclonal Rabbit anti‑Human ENO1 / Alpha Enolase Antibody (IHC, IF, WB) LS‑C331216

Antibody:
ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C331216-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha Enolase antibody LS-C331216 is an unconjugated rabbit polyclonal antibody to Alpha Enolase (ENO1) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB.
Target
Human ENO1 / Alpha Enolase
Synonyms
ENO1 | Alpha Enolase | Alpha enolase like 1 | ENO1L1 | Enolase 1 | MBP-1 | MBPB1 | MPB1 | MYC promoter-binding protein 1 | NNE | Phosphopyruvate hydratase | Alpha-enolase | Tau-crystallin | MBP1 | MPB-1 | C-myc promoter-binding protein | Enolase 1, (alpha) | Enolase-alpha | Non-neural enolase | Plasminogen-binding protein | PPH
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1). MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVG
Specificity
Human ENO1 / Alpha Enolase
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 36kDa/47kDa, while the observed MW by Western blot was 47kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ENO1 / Alpha Enolase
P06733 NM_001428 NP_001419.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024