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Catalog Number	Size	Price
LS-B3633-200	200 µl	$460

ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

ENO1 / Alpha Enolase Antibody - Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.

ENO1 / Alpha Enolase Antibody - ENO1 Antibody western blot of Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). The ENO1 antibody detected the ENO1 protein (arrow).

ENO1 / Alpha Enolase Antibody - ENO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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IHC‑plus™ Polyclonal Rabbit anti‑Human ENO1 / Alpha Enolase Antibody (aa178‑205, IHC, IF, WB) LS‑B3633

Name: IHC-plus™ ENO1 / Alpha Enolase Polyclonal Antibody Rabbit | LSBio
Brand: Lifespan BioSciences
SKU: LS-B3633-200
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C99250

Antibody:

ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal (aa178-205) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B3633-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal (aa178-205) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Alpha Enolase antibody LS-B3633 is an unconjugated rabbit polyclonal antibody to human Alpha Enolase (ENO1) (aa178-205). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human ENO1 / Alpha Enolase

Synonyms

See All ENO1 / Alpha Enolase Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Monkey, Rat, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa178-205

Specificity

This ENO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human ENO1.

Applications

IHC
IHC - Paraffin (10 µg/ml)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B3633 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3633 was determined to be 10 ug/ml.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ENO1 / Alpha Enolase

Uniprot: P06733 NCBI: NM_001428 NP_001419.1

Validation

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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About LSBio Validation process