Human ELN / Elastin

Guinea pig

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Purified elastin from human skin

• IHC - Paraffin (1:500 - 1:1000)
• Immunofluorescence (1:80)
• ELISA (1:200)
• Radioimmunoassay

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Human Elastin 100%, human Collagen Type I, II, III, IV, V <0,1%; human Fibronectin <0,1%.

PBS

0.1 ml Reconstitute in sterile distilled water.

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15502 NCBI: NM_000501 NP_000492.2