Human ELN / Elastin

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Elastin extracted and purified from human skin

Recognizes human Elastin. Crossreactivity: < 0.1% with human Collagen I, II, III, IV, V, human Fibronectin, or rat Elastin by radioimmunoassay.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (1:80)
• ELISA
• Radioimmunoassay

Immunohistochemistry: LS-B2115 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2115 was determined to be 2.5 ug/ml. Immunofluorescence: 1:80. Frozen human skin and liver tissues. Positive control: Human skin or liver.

PBS, pH 7.2, No preservatives added

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15502 NCBI: NM_000501 NP_000492.2