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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2146-50 | 50 µg (1 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Chicken ELN / Elastin Antibody (clone 10B8, IHC, IF, WB) LS‑B2146
IHC‑plus™ Monoclonal Mouse anti‑Chicken ELN / Elastin Antibody (clone 10B8, IHC, IF, WB) LS‑B2146
Note: This antibody replaces LS-C23008
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Elastin antibody LS-B2146 is an unconjugated mouse monoclonal antibody to Elastin (ELN) from chicken. It is reactive with human, mouse, bovine and other species. Validated for ELISA, IF, IHC, IP and WB. Cited in 2 publications.
Target
Chicken ELN / Elastin
Synonyms
ELN | SVAS | WBS | Elastin | Tropoelastin | WS
Host
Mouse
Reactivity
Chicken, Human, Mouse, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Clone
10B8
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Tropoelastin from 17-day old lathyric chick embryo aortae, extracted and purified according to the method of Rich and Foster, Meth. Enzymol. 82:665-673(1982).
Specificity
Recognizes chicken Elastin.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence
- Western blot
- Immunoprecipitation
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2146 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2146 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ELN / Elastin
Validation
Anti-Elastin antibody IHC of human skeletal muscle, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Elastin antibody IHC of human skeletal muscle, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ELN / Elastin Antibody (clone 10B8) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B2146 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin. Han W, Guo C, Liu Q, Yu B, Liu Z, Yang J, Deng C. Cell and tissue research. Feb;359:589-603.
A histological and immunohistochemical study on the parabronchial epithelium of the domestic fowl's (Gallus gallus domesticus) lung with special reference to its scanning and transmission electron microscopic characteristics. Ramadan M Kandyel, Hebat Allah El Basyouny, Eman E El Nahas, Fatma Madkour, Seham Haddad, Diaa Massoud, Kareem Morsy, Naglaa Madkour, Mohamed Abumandour. Microscopy research and technique. 2022 March;85:1108-1119.
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Featured Products
Species:
Bovine
Applications:
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, ELISA, Radioimmunoassay
Species:
Human, Bovine, Dog, Rabbit
Applications:
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/17/2024
Date Requested: 11/17/2024
