Target
Human EFNB2 / Ephrin B2
Synonyms
EFNB2 | Ephrin-B2 | EPLG5 | Ephrin B2 | HTK ligand | Htk-L | LERK-5 | LERK5 | Ligand of eph-related kinase 5 | HTKL
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human EFNB1/2 around the phosphorylation site of Tyrosine 330.
Specificity
Phospho-EFNB1/2 (Tyr330) Antibody detects endogenous levels of EFNB1/2 only when phosphorylated at Tyrosine 330.
Applications
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee