Target
Human EDAR
Synonyms
EDAR | Ectodysplasin-A receptor | ED1R | EDA-A1 receptor | EDA1R | DL | Downless, mouse, homolog of | ED3 | EDA3 | ECTD10A | ECTD10B | Ectodysplasin A receptor | ED5 | Downless homolog | Ectodermal dysplasia receptor | EDA-A1R | HRM1
Reactivity
Human, Mouse, Rat, Dog, Chicken
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Recombinant Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
Specificity
EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
Applications
- Western blot
- ELISA
- Surface Plasmon Resonance
Usage
EDAR123 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR can also be recognized by EDAR12 under both reducing (+DTT) and non-reducing conditions.
Presentation
PBS, 0.02% ProClin™ 300.
Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee