Target
Human E2F1
Synonyms
E2F1 | E2F transcription factor 1 | PRB-binding protein E2F-1 | RBAP-1 | RBBP3 | RBBP-3 | Transcription factor E2F1 | RBAP1 | E2F-1 | PBR3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
FITC,
HRP.
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide located between aa107-156 of human E2F1 (Q01094, NP_005216). Percent identity by BLAST analysis: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Applications
- Western blot (0.2 - 1 µg/ml)
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee