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Antibodies > DUT / DUTPase > LS-C312268
 
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Catalog Number Size Price
LS-C312268-10 10 µg $318 
LS-C312268-100 100 µg (0.5 mg/ml) $470 
DUT / DUTPase Antibody - IHC analysis of DUT using anti-DUT antibody. DUT was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DUT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DUT / DUTPase Antibody - DUT / DUTPase antibody. IHC(P): Human Intestinal Cancer Tissue.
DUT / DUTPase Antibody - Western blot analysis of DUT using anti-DUT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DUT at approximately 27KD. The expected band size for DUT is at 27KD.
DUT / DUTPase Antibody - IHC analysis of DUT using anti-DUT antibody. DUT was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DUT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DUT / DUTPase Antibody - DUT / DUTPase antibody. IHC(P): Human Intestinal Cancer Tissue.
DUT / DUTPase Antibody - Western blot analysis of DUT using anti-DUT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DUT at approximately 27KD. The expected band size for DUT is at 27KD.
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Polyclonal Rabbit anti‑Human DUT / DUTPase Antibody (aa136‑150, IHC, WB) LS‑C312268

Polyclonal Rabbit anti‑Human DUT / DUTPase Antibody (aa136‑150, IHC, WB) LS‑C312268

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Antibody:
DUT / DUTPase Rabbit anti-Human Polyclonal (aa136-150) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312268-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DUT / DUTPase Rabbit anti-Human Polyclonal (aa136-150) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
DUTPase antibody LS-C312268 is an unconjugated rabbit polyclonal antibody to DUTPase (DUT) (aa136-150) from human. It is reactive with human, mouse, monkey and other species. Validated for IHC and WB.
Target
Human DUT / DUTPase
Synonyms
DUT | Deoxyuridine triphosphatase | DUTP pyrophosphatase | DUT-N | DUTP nucleotidohydrolase | DUTPase
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human DUT(212-229 aa KKGDRIAQLICERIFYPE), different from the related rat and mouse sequences by two amino acids.
Epitope
aa136-150
Specificity
Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Reconstitute with 200µl deionized water to a working concentration of 0.5mg/ml
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DUT / DUTPase
P33316 NM_001948 NP_001939.1

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Featured Products

DUT / DUTPase Antibody - DUT antibody DUT(dUTP pyrophosphatase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human heart. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DUT / DUTPase Antibody (C‑Terminus) LS‑C80791
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
DUT / DUTPase Antibody - Western blot detection of DUT-N in HeLa cell lysate using antibody at a dilution of 1:1000.
DUT / DUTPase Antibody (aa25‑44) LS‑C673
Species: Human
Applications: Western blot
Product
DUT / DUTPase Antibody LS‑C121723
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
See all DUT / DUTPase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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