Target
Human DLK1 / Pref-1
Synonyms
DLK1 | Delta-like protein dlk | DLK-1 | Delta1 | FA1 | Dlk | Preadipocyte factor 1 | Pref-1 | Protein delta homolog 1 | PG2 | ZOG | Fetal antigen 1 | PREF1 | Secredeltin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Recombinant human DLK1 (extracellular domain).
Epitope
Extracellular Domain
Specificity
Recognizes human DLK1. Detects a band of ~42kDa by Western blot.
Applications
- Western blot (1:2000 - 1:10000)
- Flow Cytometry (1:100 - 1:500)
- ELISA (1:2000 - 1:10000)
Usage
ELISA - Direct and indirect: 1:2000-1:10000. Flow Cytometry - 1:100-1:500. Western Blot - 1:2000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee