Target
Pig DLD / Diaphorase / E3
Synonyms
DLD | Diaphorase | DLDH | Dihydrolipoamide dehydrogenase | E3 | GCSL | Lipoyl dehydrogenase | PHE3 | Lipoamide dehydrogenase | Lipoamide reductase | LAD
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Lipoamide Dehydrogenase [Porcine Heart].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]. Cross reactivity against Lipoamide Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
Usage
Anti-LIPOAMIDE DEHYDROGENASE has been assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:7000 to 1:30000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DLD / Diaphorase / E3