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| Catalog Number | Size | Price |
|---|---|---|
| LS-B194-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human DKK1 Antibody (aa250‑262, IHC, WB) LS‑B194
IHC‑plus™ Polyclonal Goat anti‑Human DKK1 Antibody (aa250‑262, IHC, WB) LS‑B194
Note: This antibody replaces LS-C20153, LS-C64743
Antibody:
DKK1 Goat anti-Human Polyclonal (aa250-262) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Bovine, Dog, Goat, Horse, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
DKK1 Goat anti-Human Polyclonal (aa250-262) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Bovine, Dog, Goat, Horse, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
DKK1 antibody LS-B194 is an unconjugated goat polyclonal antibody to DKK1 (aa250-262) from human. It is reactive with human, mouse, bovine and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human DKK1
Synonyms
DKK1 | Dickkopf-1 like | DKK-1 | Dickkopf-1 | Dickkopf-related protein 1 | Dickkopf related protein-1 | HDkk-1 | Hdkk1 | Dickkopf-like protein 1 | SK
Host
Goat
Reactivity
Human, Monkey, Mouse, Bovine, Dog, Goat, Horse, Pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence CDHHQASNSSRLHT, from the C-Terminus of protein sequence according to NP_036374.1.
Epitope
aa250-262
Specificity
Human DKK1.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B194 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B194 was determined to be 10 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DKK1
Validation
Anti-DKK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DKK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ DKK1 Antibody (aa250-262) for IHC, WB/Western LS-B194 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Molecular Characterization of Microtubule Affinity-Regulating Kinase4 from Sus scrofa and Promotion of Lipogenesis in Primary Porcine Placental Trophoblasts. Tian L, Wen A, Dong S, Yan P. International journal of molecular sciences. 2019 March;20:pii E1206.
Maternal obesity stimulates lipotoxicity and up-regulates inflammatory signaling pathways in the full-term swine placenta. Tian Liang, Xie Jinglong, Dong Shusheng, Wen Aiyou. Animal science journal = Nihon chikusan Gakkaiho. 2018 September;89:1310-1322.
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Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
