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Catalog Number	Size	Price
LS-C19112-100	100 µl (85 mg/ml)	$567

$DIABLO / SMAC Antibody - Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1$

DIABLO / SMAC Antibody - Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.

$DIABLO / SMAC Antibody - Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.$

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Polyclonal Rabbit anti‑Human DIABLO / SMAC Antibody (WB) LS‑C19112

Name: Anti-DIABLO / SMAC Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C19112-100
Price: 567.0000 USD
Availability: InStock

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Antibody:

DIABLO / SMAC Rabbit anti-Human Polyclonal Antibody

Application:

WB, IP, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$567

LS-C19112-100

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(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

DIABLO / SMAC Rabbit anti-Human Polyclonal Antibody

Application:

WB, IP, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

SMAC antibody LS-C19112 is an unconjugated rabbit polyclonal antibody to human SMAC (DIABLO). Validated for ELISA, IP and WB.

Target

Human DIABLO / SMAC

Synonyms

See All DIABLO / SMAC Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Antiserum

Modifications

Unmodified

Immunogen

This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).

Specificity

This antiserum is directed against human Smac/DIABLO and is useful in determining its presence in various assays.

Applications

Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:100)
ELISA (1:5000 - 1:20000)

Usage

This antibody reacts with human Smac/DIABLO protein by immunoblot. Lysates from human HeLa and LNCaP cells are positive for Smac/DIABLO. Other animal tissues have not been tested.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About DIABLO / SMAC

Uniprot: Q9NR28 NCBI: NM_019887 NP_063940.1