LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > DDIT3 / CHOP > LS-C801079
 
Print Page  
Catalog Number Size Price
LS-C801079-100 100 µl (1 mg/ml) $379 
LS-C801079-200 200 µl (1 mg/ml) $421 
DDIT3 / CHOP Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
DDIT3 / CHOP Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
DDIT3 / CHOP Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
DDIT3 / CHOP Antibody - Western blot analysis of CHOP phosphorylation expression in PMA treated Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
DDIT3 / CHOP Antibody - Western blot analysis of Phospho-CHOP (Ser30) expression in various lysates
DDIT3 / CHOP Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
DDIT3 / CHOP Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
DDIT3 / CHOP Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
DDIT3 / CHOP Antibody - Western blot analysis of CHOP phosphorylation expression in PMA treated Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
DDIT3 / CHOP Antibody - Western blot analysis of Phospho-CHOP (Ser30) expression in various lysates
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human DDIT3 / CHOP Antibody (phospho‑Ser30, IHC, IF, WB) LS‑C801079

Polyclonal Rabbit anti‑Human DDIT3 / CHOP Antibody (phospho‑Ser30, IHC, IF, WB) LS‑C801079

Submit Review LSBio Guarantee
Antibody:
DDIT3 / CHOP Rabbit anti-Human Polyclonal (pSer30) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801079-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DDIT3 / CHOP Rabbit anti-Human Polyclonal (pSer30) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CHOP antibody LS-C801079 is an unconjugated rabbit polyclonal antibody to CHOP (DDIT3) (pSer30) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human DDIT3 / CHOP
Synonyms
DDIT3 | CHOP10 | DDIT-3 | CHOP | GADD153 | C/EBP zeta | C/EBP-homologous protein | C/EBP-homologous protein 10 | CHOP-10
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human CHOP around the phosphorylation site of Serine 30.
Epitope
pSer30
Specificity
Phospho-CHOP (Ser30) Antibody detects endogenous levels of CHOP only when phosphorylated at Serine 30.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDIT3 / CHOP
P35638 NM_004083 NP_004074.2

Publications (0)

Submit a Publication for LS-C801079

Customer Reviews (0)

Submit a review for LS-C801079

Featured Products

Product
DDIT3 / CHOP Antibody (aa120‑149, APC) LS‑C234466
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
DDIT3 / CHOP Antibody - Anti-DDIT3 / CHOP antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ DDIT3 / CHOP Antibody (clone 3H7) LS‑B11229
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
DDIT3 / CHOP Antibody - Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.
DDIT3 / CHOP Antibody (aa15‑64) LS‑C117859
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
DDIT3 / CHOP Antibody - Detection limit for recombinant GST tagged DDIT3 is approximately 0.03 ng/ml as a capture antibody.
DDIT3 / CHOP Antibody (clone 2G3) LS‑C197035
Species: Human
Applications: Western blot, ELISA
DDIT3 / CHOP Antibody - Fluorescent image of HeLa cell stained with DDIT3 Antibody (C-term A135). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
DDIT3 / CHOP Antibody (aa120‑149) LS‑C167320
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Flow Cytometry
DDIT3 / CHOP Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GADD153 Antibody. The picture on the right is blocked with the synthesized peptide.
DDIT3 / CHOP Antibody (aa91‑140) LS‑C118458
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all DDIT3 / CHOP Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com