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Catalog Number Size Price
LS-C167320-400 400 µl $393 
DDIT3 / CHOP Antibody - Fluorescent image of HeLa cell stained with DDIT3 Antibody (C-term A135). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
DDIT3 / CHOP Antibody - DDIT3 Antibody (C-term A135) western blot of mouse testis tissue lysates (35 ug/lane). The DDIT3 antibody detected the DDIT3 protein (arrow).
DDIT3 / CHOP Antibody - DDIT3 Antibody (C-term A135) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
DDIT3 / CHOP Antibody - Fluorescent image of HeLa cell stained with DDIT3 Antibody (C-term A135). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
DDIT3 / CHOP Antibody - DDIT3 Antibody (C-term A135) western blot of mouse testis tissue lysates (35 ug/lane). The DDIT3 antibody detected the DDIT3 protein (arrow).
DDIT3 / CHOP Antibody - DDIT3 Antibody (C-term A135) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human DDIT3 / CHOP Antibody (aa120‑149, IF, WB) LS‑C167320

Polyclonal Rabbit anti‑Human DDIT3 / CHOP Antibody (aa120‑149, IF, WB) LS‑C167320

Antibody:
DDIT3 / CHOP Rabbit anti-Human Polyclonal (aa120-149) Antibody
Application:
IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C167320-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
DDIT3 / CHOP Rabbit anti-Human Polyclonal (aa120-149) Antibody
Application:
IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CHOP antibody LS-C167320 is an unconjugated rabbit polyclonal antibody to CHOP (DDIT3) (aa120-149) from human. It is reactive with human and mouse. Validated for Flow, IF and WB.
Target
Human DDIT3 / CHOP
Synonyms
DDIT3 | CHOP10 | DDIT-3 | CHOP | GADD153 | C/EBP zeta | C/EBP-homologous protein | C/EBP-homologous protein 10 | CHOP-10
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Hamster, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa120-149
Specificity
This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDIT3 / CHOP
P35638 NM_004083 NP_004074.2

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Requested From: United States
Date Requested: 11/27/2024