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Antibodies > DDIT3 / CHOP > LS-C353971
 
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Catalog Number Size Price
LS-C353971-100 100 µl (1 mg/ml) $299 
DDIT3 / CHOP Antibody - Immunohistochemical analysis of GADD153 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DDIT3 / CHOP Antibody - Immunofluorescent analysis of GADD153 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DDIT3 / CHOP Antibody - Western blot analysis of GADD153 expression in Jurkat (A); HeLa (B) whole cell lysates.
DDIT3 / CHOP Antibody - Immunohistochemical analysis of GADD153 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DDIT3 / CHOP Antibody - Immunofluorescent analysis of GADD153 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DDIT3 / CHOP Antibody - Western blot analysis of GADD153 expression in Jurkat (A); HeLa (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human DDIT3 / CHOP Antibody (N‑Terminus, IHC, IF, WB) LS‑C353971

Polyclonal Rabbit anti‑Human DDIT3 / CHOP Antibody (N‑Terminus, IHC, IF, WB) LS‑C353971

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Antibody:
DDIT3 / CHOP Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353971-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DDIT3 / CHOP Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
CHOP antibody LS-C353971 is an unconjugated rabbit polyclonal antibody to CHOP (DDIT3) (N-Terminus) from human. It is reactive with human, monkey and pig. Validated for ICC, IF, IHC, IP and WB.
Target
Human DDIT3 / CHOP
Synonyms
DDIT3 | CHOP10 | DDIT-3 | CHOP | GADD153 | C/EBP zeta | C/EBP-homologous protein | C/EBP-homologous protein 10 | CHOP-10
Host
Rabbit
Reactivity
Human, Monkey, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human GADD153.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of GADD153 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDIT3 / CHOP
P35638 NM_004083 NP_004074.2

Publications (0)

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Featured Products

Product
DDIT3 / CHOP Antibody (aa120‑149, APC) LS‑C234466
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
DDIT3 / CHOP Antibody - Anti-DDIT3 / CHOP antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ DDIT3 / CHOP Antibody (clone 3H7) LS‑B11229
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
DDIT3 / CHOP Antibody - Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.
DDIT3 / CHOP Antibody (aa15‑64) LS‑C117859
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
DDIT3 / CHOP Antibody - Detection limit for recombinant GST tagged DDIT3 is approximately 0.03 ng/ml as a capture antibody.
DDIT3 / CHOP Antibody (clone 2G3) LS‑C197035
Species: Human
Applications: Western blot, ELISA
DDIT3 / CHOP Antibody - Fluorescent image of HeLa cell stained with DDIT3 Antibody (C-term A135). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
DDIT3 / CHOP Antibody (aa120‑149) LS‑C167320
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Flow Cytometry
DDIT3 / CHOP Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GADD153 Antibody. The picture on the right is blocked with the synthesized peptide.
DDIT3 / CHOP Antibody (aa91‑140) LS‑C118458
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all DDIT3 / CHOP Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
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