Target
Human DCX / Doublecortin
Synonyms
DCX | DBCN | Doublecortin | Doublin | Doublecortex | Lis-X | SCLH | LISX | XLIS | Lissencephalin-X
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence DRSHCQSLRFHQN, from the internal region of the protein sequence according to NP_000546.2.
Epitope
Amino Acids 69-81
Specificity
This antibody is expected to recognize reported isoform a (NP_000546.2) only.
Applications
- Immunofluorescence (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee