Target
Mouse DCX / Doublecortin
Synonyms
DCX | DBCN | Doublecortin | Doublin | Doublecortex | Lis-X | SCLH | LISX | XLIS | Lissencephalin-X
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KASPTPQKTSAKSPG, from the internal region of the protein sequence according to NP_000546.2NP_835365.1NP_835364.1NP_835366.1.
Specificity
This antibody is expected to recognise isoform a (NP_000546.2), isoform b (NP_835365.1), and isoform c (NP_835364.1 and NP_835366.1).
Applications
- Immunofluorescence (5 µg/ml)
- Western blot (0.00 - 0.01 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to Mouse NP_001103692.1). Recommended concentration: 0.003-0.01 ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee