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Antibodies > DCK / Deoxycytidine kinase > LS-C662728
 
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Catalog Number Size Price
LS-C662728-10 10 µg $318 
LS-C662728-100 100 µg $470 
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - Western blot analysis of DCK using anti-DCK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DCK at approximately 30KD. The expected band size for DCK is at 30KD.
DCK / Deoxycytidine kinase Antibody - Western blot - Anti-DCK Picoband antibody
DCK / Deoxycytidine kinase Antibody - Flow Cytometry analysis of U20S cells using anti-DCK antibody. Overlay histogram showing U20S cells stained with anti-DCK antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DCK Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - IHC analysis of DCK using anti-DCK antibody. DCK was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DCK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DCK / Deoxycytidine kinase Antibody - Western blot analysis of DCK using anti-DCK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DCK at approximately 30KD. The expected band size for DCK is at 30KD.
DCK / Deoxycytidine kinase Antibody - Western blot - Anti-DCK Picoband antibody
DCK / Deoxycytidine kinase Antibody - Flow Cytometry analysis of U20S cells using anti-DCK antibody. Overlay histogram showing U20S cells stained with anti-DCK antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DCK Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human DCK / Deoxycytidine kinase Antibody (WB) LS‑C662728

Polyclonal Rabbit anti‑Human DCK / Deoxycytidine kinase Antibody (WB) LS‑C662728

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Antibody:
DCK / Deoxycytidine kinase Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662728-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DCK / Deoxycytidine kinase Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Deoxycytidine kinase antibody LS-C662728 is an unconjugated rabbit polyclonal antibody to human Deoxycytidine kinase (DCK). Validated for ELISA and WB.
Target
Human DCK / Deoxycytidine kinase
Synonyms
DCK | Deoxynucleoside kinase | Deoxycytidine kinase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human DCK recombinant protein (Position: E17-L260).
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DCK / Deoxycytidine kinase
P27707 NM_000788 NP_000779.1

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DCK / Deoxycytidine kinase Antibody - DCK antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DCK / Deoxycytidine kinase Antibody - DCK Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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DCK / Deoxycytidine kinase Antibody - DCK antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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See all DCK / Deoxycytidine kinase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/1/2024
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