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Catalog Number Size Price
LS-C344043-10 10 µg $318 
LS-C344043-100 100 µg (0.5 mg/ml) $470 
CYP2E1 Antibody - CYP2E1 antibody IHC-paraffin: Rat Liver Tissue.
CYP2E1 Antibody - CYP2E1 antibody IHC-paraffin: Mouse Liver Tissue.
CYP2E1 Antibody - Western blot analysis of CYP2E1 using anti-CYP2E1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat liver tissue lysate,lane 2: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 55KD. The expected band size for CYP2E1 is at 57KD.
CYP2E1 Antibody - CYP2E1 antibody Western blot. All lanes: Anti CYP2E1 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Predicted band size: 55 kD. Observed band size: 65 kD.
CYP2E1 Antibody - CYP2E1 antibody Western blot. All lanes: Anti CYP2E1 at 0.5 ug/ml. WB: Recombinant Human CYP2E1 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
CYP2E1 Antibody - CYP2E1 antibody IHC-paraffin: Rat Liver Tissue.
CYP2E1 Antibody - CYP2E1 antibody IHC-paraffin: Mouse Liver Tissue.
CYP2E1 Antibody - Western blot analysis of CYP2E1 using anti-CYP2E1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat liver tissue lysate,lane 2: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 55KD. The expected band size for CYP2E1 is at 57KD.
CYP2E1 Antibody - CYP2E1 antibody Western blot. All lanes: Anti CYP2E1 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Predicted band size: 55 kD. Observed band size: 65 kD.
CYP2E1 Antibody - CYP2E1 antibody Western blot. All lanes: Anti CYP2E1 at 0.5 ug/ml. WB: Recombinant Human CYP2E1 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
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Polyclonal Rabbit anti‑Human CYP2E1 Antibody (aa1‑310, IHC, WB) LS‑C344043

Polyclonal Rabbit anti‑Human CYP2E1 Antibody (aa1‑310, IHC, WB) LS‑C344043

Note: This antibody replaces LS-C388320
Antibody:
CYP2E1 Rabbit anti-Human Polyclonal (aa1-310) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344043-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CYP2E1 Rabbit anti-Human Polyclonal (aa1-310) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CYP2E1 antibody LS-C344043 is an unconjugated rabbit polyclonal antibody to CYP2E1 (aa1-310) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CYP2E1
Synonyms
CYP2E1 | 4-nitrophenol 2-hydroxylase | CPE1 | Cytochrome p450 IIe1 | Cytochrome P450-J | p450C2E | p450-J | CYP2E | CYPIIE1 | Cytochrome P450 2E1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively.
Epitope
aa1-310
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP2E1
P05181 NM_000773 NP_000764.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/8/2024