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Catalog Number	Size	Price
LS-C313045-10	10 µg	$318
LS-C313045-100	100 µg (0.5 mg/ml)	$470

CYP2E1 Antibody - IHC analysis of CYP2E using anti-CYP2E antibody. CYP2E was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CYP2E Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CYP2E1 Antibody - IHC analysis of CYP2E using anti-CYP2E antibody. CYP2E was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CYP2E Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CYP2E1 Antibody - CYP2E1 antibody. IHC(P): Rat Liver Tissue.

CYP2E1 Antibody - Western blot analysis of CYP2E1 using anti-CYP2E1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.

CYP2E1 Antibody - WB of CYP2E1 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Mouse Liver Tissue Lysate.

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Polyclonal Rabbit anti‑Mouse CYP2E1 Antibody (aa94‑112, IHC, WB) LS‑C313045

Name: Anti-CYP2E1 Antibody | Rabbit anti-Mouse Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313045-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C388322

Antibody:

CYP2E1 Rabbit anti-Mouse Polyclonal (aa94-112) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C313045-10

Toll Free North America

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For Research Use Only

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Overview

Antibody:

CYP2E1 Rabbit anti-Mouse Polyclonal (aa94-112) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CYP2E1 antibody LS-C313045 is an unconjugated rabbit polyclonal antibody to CYP2E1 (aa94-112) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.

Target

Mouse CYP2E1

Synonyms

See All CYP2E1 Antibodies

Host

Rabbit

Reactivity

Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of mouse CYP2E1 (94-112 aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.

Epitope

aa94-112

Specificity

Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CYP2E1

Uniprot: P05181 NCBI: NM_000773 NP_000764.1