LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > CXADR > LS-C313489
 
Print Page  
Catalog Number Size Price
LS-C313489-10 10 µg $318 
LS-C313489-100 100 µg $470 
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U-937whole cell lysates(negative), Lane 6: human THP-1whole cell lysates(negative). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - WB of CXADR antibody. Lane 1: Rat Pancreas Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Heart Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Lane 6: COLO320 Cell Lysate.
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U-937whole cell lysates(negative), Lane 6: human THP-1whole cell lysates(negative). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - WB of CXADR antibody. Lane 1: Rat Pancreas Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Heart Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Lane 6: COLO320 Cell Lysate.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human CXADR Antibody (aa313‑328, WB) LS‑C313489

Polyclonal Rabbit anti‑Human CXADR Antibody (aa313‑328, WB) LS‑C313489

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C388129
Antibody:
CXADR Rabbit anti-Human Polyclonal (aa313-328) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313489-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CXADR Rabbit anti-Human Polyclonal (aa313-328) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CXADR antibody LS-C313489 is an unconjugated rabbit polyclonal antibody to CXADR (aa313-328) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human CXADR
Synonyms
CXADR | CAR4/6 | Coxsackie-adenovirus receptor | CVB3-binding protein | CAR | HCAR | HCVADR
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Coxsackie Adenovirus Receptor(313-328 aa YSKTQYNQVPSEDFER), identical to the related rat and mouse sequences.
Epitope
aa313-328
Specificity
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. .
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CXADR
P78310 NM_001338 NP_001329.1

Publications (0)

Submit a Publication for LS-C313489

Customer Reviews (0)

Submit a review for LS-C313489

Featured Products

Product
CXADR Antibody LS‑C21204
Species: Human, Mouse, Rat
Applications: ICC, Western blot
CXADR Antibody - Anti-CXADR antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ CXADR Antibody (Internal) LS‑A8845
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin
CXADR Antibody - Anti-CXADR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
IHC‑plus™ CXADR Antibody LS‑B8019
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
CXADR Antibody - Western blot of anti-CXADR Antibody in NCI-H460 cell line lysates (35 ug/lane). CXADR(arrow) was detected using the purified antibody.
CXADR Antibody (aa132‑161) LS‑C97350
Species: Human
Applications: Western blot, Flow Cytometry
CXADR Antibody - Anti-CXADR antibody IHC of human stomach. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ CXADR Antibody (Internal) LS‑A8846
Species: Human
Applications: IHC, IHC - Paraffin
CXADR Antibody - Anti-CXADR antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ CXADR Antibody (aa1‑50) LS‑B8702
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CXADR Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com