Target
Human CXADR
Synonyms
CXADR | CAR4/6 | Coxsackie-adenovirus receptor | CVB3-binding protein | CAR | HCAR | HCVADR
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Native human Coxsackie virus adenovirus receptor protein.
Specificity
Recognizes human CAR at Mr 46kD. Species cross-reactivity: Does not cross-react with mouse.
Applications
- ICC (1 - 2 µg/ml)
- Western blot (0.2 - 2 µg/ml)
Usage
Suitable for Western Blot and Immunocytochemistry. Proteins were visualized using an goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 1-2 ug/ml shows positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. Positive control: Lung tissue.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee