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Catalog Number	Size	Price
LS-C353976-30	30 µl	$263
LS-C353976-50	50 µl	$279
LS-C353976-100	100 µl (1 mg/ml)	$315
LS-C353976-200	200 µl	$379

CTTN / Cortactin Antibody - Immunohistochemical analysis of Cortactin (pY466) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

CTTN / Cortactin Antibody - Immunofluorescent analysis of Cortactin (pY466) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CTTN / Cortactin Antibody - Western blot analysis of Cortactin (pY466) expression in HeLa UV-treated (A); NIH3T3 H2O2-treated (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human CTTN / Cortactin Antibody (phospho‑Tyr466, IHC, IF, WB) LS‑C353976

Name: Anti-CTTN / Cortactin Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C353976-30
Price: 263.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CTTN / Cortactin Rabbit anti-Human Polyclonal (pTyr466) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$263

LS-C353976-30

$279

LS-C353976-50

$315

LS-C353976-100

$379

LS-C353976-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CTTN / Cortactin Rabbit anti-Human Polyclonal (pTyr466) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

Cortactin antibody LS-C353976 is an unconjugated rabbit polyclonal antibody to Cortactin (CTTN) (pTyr466) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC, IP and WB.

Target

Human CTTN / Cortactin

Synonyms

See All CTTN / Cortactin Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Cortactin (pY466).

Epitope

pTyr466

Specificity

Recognizes endogenous levels of Cortactin (pY466) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CTTN / Cortactin

Uniprot: Q14247 NCBI: NM_005231 NP_005222.2