Human CTNNB1 / Beta Catenin

Rabbit

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide corresponding to the consensus GSK3 phosphorylation site (aa29-49, CGG-SYLDSGIHSGATTTAPSLSGK) of human b-Catenin (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%); Stickleback (90%).

aa29-49

Recognizes b-Catenin, Mr 92kD. Species cross-reactivity: Human, bovine, rat, rabbit and mouse.

• ICC (1 - 5 µg/ml)
• Western blot (0.1 - 2 µg/ml)
• Immunoprecipitation

WB: Detects b-catenin in an A431 total cell lysate. A431 total cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-b-Catenin (0.1 ug/ml). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. ICC: Demonstrates positive immunostaining for b-catenin at cell-cell junctions of A431 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. IP: 4ug immunoprecipitates b-Catenin from 500ug of A431 membrane fraction lysate.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35222 NCBI: NM_001904 NP_001895.1