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Catalog Number	Size	Price
LS-C661902-10	10 µg	$318
LS-C661902-100	100 µg	$470

CTLA4 / CD152 Antibody - IHC analysis of CTLA4 using anti-CTLA4 antibody. CTLA4 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTLA4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CTLA4 / CD152 Antibody - IHC analysis of CTLA4 using anti-CTLA4 antibody. CTLA4 was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTLA4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CTLA4 / CD152 Antibody - IHC analysis of CTLA4 using anti-CTLA4 antibody. CTLA4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTLA4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CTLA4 / CD152 Antibody - Western blot analysis of CTLA4 using anti-CTLA4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD.

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Polyclonal Rabbit anti‑Mouse CTLA4 / CD152 Antibody (aa36‑161, IHC, WB) LS‑C661902

Name: Anti-CTLA4 / CD152 Antibody | Rabbit anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C661902-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CTLA4 / CD152 Rabbit anti-Mouse Polyclonal (aa36-161) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C661902-10

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Overview

Antibody:

CTLA4 / CD152 Rabbit anti-Mouse Polyclonal (aa36-161) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CD152 antibody LS-C661902 is an unconjugated rabbit polyclonal antibody to CD152 (CTLA4) (aa36-161) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.

Target

Mouse CTLA4 / CD152

Synonyms

See All CTLA4 / CD152 Antibodies

Host

Rabbit

Reactivity

Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived mouse CTLA4 recombinant protein (Position: E36-D161). Mouse CTLA4 shares 67.5% amino acid (aa) sequence identity with human CTLA4.

Epitope

aa36-161

Specificity

Widely expressed with highest levels in lymphoid tissues. .

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CTLA4 / CD152

Uniprot: P16410 NCBI: NM_005214 NP_005205.2