Human CSPG4 / NG2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein A purified

Azide-free

Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.

Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.

• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry
• Functional Assay

Suitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Immunofluorescence Microscopy: Cells in culture. Immunoprecipitation: 1 ug/1E6 cells. FACS analysis: 1-2 ug/1E6 cells. Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.6. No preservative added. Glycerol free.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q6UVK1 NCBI: NM_001897 NP_001888.2