Human CREBBP / CREB Binding Protein

Mouse

Human, Monkey, Mouse, Rat, Mink (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Azide-free

E1A affinity purified p300 protein.

Recognizes human p300 and CBP. p300 and CBP differ by three noncontiguous residues within the 21aa epitope region. A difference that does not detectably affect the reactivity of LS-C12614. Species cross-reactivity: monkey, mink, mouse and rat.

• IHC
• IHC - Paraffin
• Immunofluorescence
• Western blot

Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Tonsil. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.4

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q92793 NCBI: NM_004380 NP_004371.2