Target
Guinea pig Complement C3c
Reactivity
Guinea pig, Pig
(tested or 100% immunogen sequence identity)
Immunogen
The protein is isolated and purified from pooled normal guinea pig serum by precipitation techniques, followed by chromatographical methods.
Specificity
Guinea pig Complement C3, C3c subunit. In IEP against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma.