Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Immunogen
C3c isolated and purified from pooled normal mouse serum.
Specificity
Native and activated mouse C3 and C3c. In immunoelectrophoresis against fresh mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other protein components of mouse serum or plasma.