Mammal Collagen III

Rabbit

Mammal, Human, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Affinity chromatography

Unmodified

Collagen Type III from human and bovine placenta.

Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

• IHC (1:200 - 1:1000)
• Western blot (1:1000 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:10000 - 1:50000)

Applications should be user optimized.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.25 M NaCl, 0.01% Thimerosal

Reconstitute in 50µL of deionized water.

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.