Bovine COL1A1 / Collagen I Alpha 1

Rabbit

Bovine, Human, Pig, Sheep, Cow (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Purified collagen type I from bovine skin

• IHC - Paraffin (1:1000)
• Immunofluorescence (1:80)
• Western blot
• ELISA (1:400)
• Radioimmunoassay

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type I 100%, bovine collagen II, IX, XI <0.1%, sheep collagen type I 50 % (IHC estimated), bovine fibronectin <0.1%, human collagen type I <2%; chicken collagen type I <0.1% (estimates except sheep collagen I from solid phase RIA at a 1:500 dilution ).

Lyophilized from PBS

Reconstitute in 100 µl of distilled water.

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02452 NCBI: NM_000088 NP_000079.2