Human CLDN1 / Claudin 1

Rabbit

Human, Rat, Dog (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of human Claudin-1 protein.

C-Terminus

Recognizes the ~22kD Claudin-1 protein, and does not cross-react with Claudin-3. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates and rat kidney homogenates by Western Blot and immunofluorescence, and with human skin tissue by immunohistochemistry.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• Immunofluorescence (5 - 10 µg/ml)
• Western blot (1 - 2 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA (Immunogen): 0.1-1 ug/ml. Western Blot (Canine, Human): 1-2 ug/ml. Immunofluorescence (Rat): 5-10 ug/ml. Immunohistochemistry (Human): 5-10 ug/ml; For best results in IHC with formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining.

PBS, pH 7.4, 0.05% Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95832 NCBI: NM_021101 NP_066924.1