LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > CHEK1 / CHK1 > LS-C352014
 
Print Page  
Catalog Number Size Price
LS-C352014-30 30 µl $255 
LS-C352014-50 50 µl $271 
LS-C352014-100 100 µl (1 mg/ml) $299 
LS-C352014-200 200 µl $351 
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CHEK1 / CHK1 Antibody - Immunofluorescent analysis of CHK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CHEK1 / CHK1 Antibody - Western blot analysis of CHK1 expression in HeLa (A); mouse kidney (B); rat heart (C) whole cell lysates.
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CHEK1 / CHK1 Antibody - Immunofluorescent analysis of CHK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CHEK1 / CHK1 Antibody - Western blot analysis of CHK1 expression in HeLa (A); mouse kidney (B); rat heart (C) whole cell lysates.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (Internal, IHC, IF, WB) LS‑C352014

Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (Internal, IHC, IF, WB) LS‑C352014

Submit Review LSBio Guarantee
Antibody:
CHEK1 / CHK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C352014-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CHEK1 / CHK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CHK1 antibody LS-C352014 is an unconjugated rabbit polyclonal antibody to CHK1 (CHEK1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human CHEK1 / CHK1
Synonyms
CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human CHK1.
Epitope
Internal
Specificity
Recognizes endogenous levels of CHK1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK1 / CHK1
O14757 NM_001274 NP_001265.2

Publications (0)

Submit a Publication for LS-C352014

Customer Reviews (0)

Submit a review for LS-C352014

Featured Products

CHEK1 / CHK1 Antibody - IHC of p-Chk1 (S345) pAb in paraffin-embedded human breast carcinoma tissue.
CHEK1 / CHK1 Antibody (phospho‑Ser345) LS‑C177888
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
CHEK1 / CHK1 Antibody - Anti-Chk1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHEK1 / CHK1 Antibody (aa192‑241) LS‑B932
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
CHEK1 / CHK1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
CHEK1 / CHK1 Antibody (phospho‑Ser317) LS‑C97441
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Dot Blot
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 (pS345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CHEK1 / CHK1 Antibody (phospho‑Ser345) LS‑C358948
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
CHEK1 / CHK1 Antibody
IHC‑plus™ CHEK1 / CHK1 Antibody LS‑B10485
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
CHEK1 / CHK1 Antibody - Immunofluorescence of monoclonal antibody to CHEK1 on HeLa cell . [antibody concentration 10 ug/ml]
CHEK1 / CHK1 Antibody (clone 2G3) LS‑C196906
Species: Human
Applications: Immunofluorescence, ELISA
See all CHEK1 / CHK1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/10/2025
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

Orders:
customerservice@vectorlabs.com

For technical assistance:
Technical.Support@LSBio.com