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Antibodies > CHAT > LS-C829397
 
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Catalog Number Size Price
LS-C829397-100 100 µl $393 
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:500 incubated at 4°C overnight.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1:250, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
CHAT Antibody - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:500 incubated at 4°C overnight.
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Polyclonal Rabbit‑New Zealand White anti‑Mouse CHAT Antibody (IHC, WB) LS‑C829397

Polyclonal Rabbit‑New Zealand White anti‑Mouse CHAT Antibody (IHC, WB) LS‑C829397

Submit Review LSBio Guarantee
Antibody:
CHAT Rabbit-New Zealand White anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB
Reactivity:
Mouse, Human, Marmoset, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C829397-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CHAT Rabbit-New Zealand White anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB
Reactivity:
Mouse, Human, Marmoset, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CHAT antibody LS-C829397 is an unconjugated rabbit-new zealand white polyclonal antibody to CHAT from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Mouse CHAT
Synonyms
CHAT | Choline acetylase | Choline acetyltransferase | Choline O-acetyltransferase | CMS1A2 | CHOACTASE | CMS1A
Host
Rabbit-New Zealand White
Reactivity
Mouse, Human, Marmoset, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
A synthetic peptide from mouse ChAT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
Applications
  • IHC - Paraffin (1:250)
  • Western blot (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized. Whole Serum.
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol (1:1) may be added for an additional stability.
Storage
Store at 2°C to 8°C for short term storage, or at -20°C for long term storage. Stable for up to 12 months after reconstitution. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHAT
P28329 NM_020549 NP_065574.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
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