Pig CHAT

Mouse

Pig, Human, Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

1.B3.9B3

Unconjugated

Purified

Unmodified

Highly purified ChAT obtained from porcine brain

Recognizes choline acetyltransferase (ChAT)-positive cells. Species cross-reactivity: Human, Rat and Porcine.

• IHC
• IHC - 4% paraformaldehyde (10 - 20 µg/ml)
• Western blot
• ELISA

Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4% paraformaldehyde/10% formaldehyde for 1 week at 4°C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10%, 20% and 30%). Prepare 50um sections and store in a cryoprotective solution at -15°C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections.

PBS, pH 7.6, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P28329 NCBI: NM_020549 NP_065574.3