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Catalog Number Size Price
LS-C748304-20 20 µl $275 
LS-C748304-100 100 µl $389 
CHAT Antibody - Immunofluorescence analysis of NIH/3T3 cells using CHAT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CHAT Antibody - Western blot analysis of extracts of various cell lines, using CHAT antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
CHAT Antibody - Immunofluorescence analysis of NIH/3T3 cells using CHAT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CHAT Antibody - Western blot analysis of extracts of various cell lines, using CHAT antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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Polyclonal Rabbit anti‑Human CHAT Antibody (IF, WB) LS‑C748304

Polyclonal Rabbit anti‑Human CHAT Antibody (IF, WB) LS‑C748304

Antibody:
CHAT Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C748304-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CHAT Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CHAT antibody LS-C748304 is an unconjugated rabbit polyclonal antibody to CHAT from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Target
Human CHAT
Synonyms
CHAT | Choline acetylase | Choline acetyltransferase | Choline O-acetyltransferase | CMS1A2 | CHOACTASE | CMS1A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1). VLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:1000 - 1:2000)
Usage
The predicted MW is 70kDa/74kDa/82kDa, while the observed MW by Western blot was 70kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHAT
P28329 NM_020549 NP_065574.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/27/2024