Polyclonal Goat anti‑Mouse CHAT Antibody (aa629‑642, IHC, WB) LS‑C34669
Polyclonal Goat anti‑Mouse CHAT Antibody (aa629‑642, IHC, WB) LS‑C34669
Note: This antibody replaces LS-C79269
Antibody:
CHAT Goat anti-Mouse Polyclonal (aa629-642) Antibody
Application:
IHC-Fr, WB, Peptide-ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
CHAT Goat anti-Mouse Polyclonal (aa629-642) Antibody
Application:
IHC-Fr, WB, Peptide-ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
CHAT antibody LS-C34669 is an unconjugated goat polyclonal antibody to mouse CHAT (aa629-642). Validated for IHC, Peptide-ELISA and WB.
Target
Mouse CHAT
Synonyms
CHAT | Choline acetylase | Choline acetyltransferase | Choline O-acetyltransferase | CMS1A2 | CHOACTASE | CMS1A
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KERARGPTKPSNLD, from the C-Terminus of protein sequence according to NP_034021.1.
Specificity
Mouse CHAT. This antibody is NOT expected to cross-react to Human CHAT.
Applications
- IHC - Frozen (1:2000)
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75-80 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3 kDa according to NP_034021.1). Recommended concentration: 0.2-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
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Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024