Human CEACAM8 / CD66b

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC3C5

Unconjugated. Also available conjugated with Biotin, FITC.

Protein A purified

Unmodified. Also available Preservative Free.

Recombinant soluble human CD66b.

Antibody from clone ANC3C5 binds to CD66b on the cell surface of human PMN in Flow cytometry and works as an EIA capture or detection reagent for recombinant CD66b when paired with clone ANC4A4.

• Flow Cytometry
• ELISA

Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD66b antibody(ANC3C5) at 1 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.31 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Binding was blocked when reagent was pre incubated with a 20-fold excess of recombinant CD66b.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31997 NCBI: NM_001816 NP_001807.2