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Antibodies > CDH1 / E Cadherin > LS-C796924
 
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LS-C796924-100 100 µl (1 mg/ml) $493 
CDH1 / E Cadherin Antibody - IHC staining of FFPE human breast cancer using anti-CDH1 clone UMAB184 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CDH1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH1. (1:500)
CDH1 / E Cadherin Antibody - Western blot analysis of extracts. (35ug) from 6 cell lines lysates by using anti-CDH1 monoclonal antibody. (1:500)
CDH1 / E Cadherin Antibody - Western blot analysis of extracts. (35ug) from 2 tissue lysates by using anti-CDH1 monoclonal antibody. (1:500)
CDH1 / E Cadherin Antibody - Flow cytometric analysis of living T47D cells, using anti-CDH1 antibody Red), compared to an isotype control. (green), and a PBS control. (blue). (1:100)
CDH1 / E Cadherin Antibody - Flow cytometric analysis of living 293T cells transfected with CDH1 overexpression plasmid Red)/empty vector Blue) using anti-CDH1 antibody. Cells incubated with a non-specific antibody. (Green) were used as isotype control. (1:100)
CDH1 / E Cadherin Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CDH1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)
CDH1 / E Cadherin Antibody - IHC staining of FFPE human breast cancer using anti-CDH1 clone UMAB184 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CDH1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH1. (1:500)
CDH1 / E Cadherin Antibody - Western blot analysis of extracts. (35ug) from 6 cell lines lysates by using anti-CDH1 monoclonal antibody. (1:500)
CDH1 / E Cadherin Antibody - Western blot analysis of extracts. (35ug) from 2 tissue lysates by using anti-CDH1 monoclonal antibody. (1:500)
CDH1 / E Cadherin Antibody - Flow cytometric analysis of living T47D cells, using anti-CDH1 antibody Red), compared to an isotype control. (green), and a PBS control. (blue). (1:100)
CDH1 / E Cadherin Antibody - Flow cytometric analysis of living 293T cells transfected with CDH1 overexpression plasmid Red)/empty vector Blue) using anti-CDH1 antibody. Cells incubated with a non-specific antibody. (Green) were used as isotype control. (1:100)
CDH1 / E Cadherin Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CDH1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)
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Monoclonal Mouse anti‑Human CDH1 / E Cadherin Antibody (clone UMAB184, IHC, IF, WB) LS‑C796924

Monoclonal Mouse anti‑Human CDH1 / E Cadherin Antibody (clone UMAB184, IHC, IF, WB) LS‑C796924

Submit Review LSBio Guarantee
Antibody:
CDH1 / E Cadherin Mouse anti-Human Monoclonal (UMAB184) Antibody
Application:
IHC, IF, WB, Flo, PMA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Carrier-free
Price
Catalog Number
$493
LS-C796924-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDH1 / E Cadherin Mouse anti-Human Monoclonal (UMAB184) Antibody
Application:
IHC, IF, WB, Flo, PMA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Carrier-free

Specifications

Description
E Cadherin antibody LS-C796924 is an unconjugated mouse monoclonal antibody to human E Cadherin (CDH1). Validated for Flow, IF, IHC, PMA and WB.
Target
Human CDH1 / E Cadherin
Synonyms
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
UMAB184
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified. Also available Carrier-free.
Immunogen
The extracellular domain of human CDH1 (NP_004351) produced in human cells.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100 - 1:200)
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • Protein Microarray
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDH1 / E Cadherin
P12830 NM_004360 NP_004351.1

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IHC‑plus™ CDH1 / E Cadherin Antibody (clone 3F4) LS‑B4297
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Applications: IHC, IHC - Paraffin, Western blot
CDH1 / E Cadherin Antibody - Anti-E Cadherin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ CDH1 / E Cadherin Antibody (aa1‑50) LS‑B5995
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CDH1 / E Cadherin Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/27/2025
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