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Antibodies > CDH1 / E Cadherin > LS-C796922
 
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Catalog Number Size Price
LS-C796922-100 100 µg $539 
CDH1 / E Cadherin Antibody - IHC staining of FFPE human breast cancer using anti-CDH1 clone UMAB184 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.
CDH1 / E Cadherin Antibody - Western blot of human tissue lysates. (15ug) from 5 different tissues. (1: Liver, 2: Ovary, 3: Thyroid, 4: Colon, 5: Spleen ). Diluation: 1:500.
CDH1 / E Cadherin Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
CDH1 / E Cadherin Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CDH1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)
CDH1 / E Cadherin Antibody - IHC staining of FFPE human breast cancer using anti-CDH1 clone UMAB184 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120C for 3 min. (1:200)
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
CDH1 / E Cadherin Antibody - Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 clone UMAB184 at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.
CDH1 / E Cadherin Antibody - Western blot of human tissue lysates. (15ug) from 5 different tissues. (1: Liver, 2: Ovary, 3: Thyroid, 4: Colon, 5: Spleen ). Diluation: 1:500.
CDH1 / E Cadherin Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
CDH1 / E Cadherin Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CDH1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)
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Monoclonal Mouse anti‑Human CDH1 / E Cadherin Antibody (clone UMAB184, Carrier‑free, IHC, IF, WB) LS‑C796922

Monoclonal Mouse anti‑Human CDH1 / E Cadherin Antibody (clone UMAB184, Carrier‑free, IHC, IF, WB) LS‑C796922

Submit Review LSBio Guarantee
Antibody:
CDH1 / E Cadherin Mouse anti-Human Monoclonal (Carrier-free) (UMAB184) Antibody
Application:
IHC, IF, WB, Flo, PMA
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified
Price
Catalog Number
$539
LS-C796922-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDH1 / E Cadherin Mouse anti-Human Monoclonal (Carrier-free) (UMAB184) Antibody
Application:
IHC, IF, WB, Flo, PMA
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified

Specifications

Description
E Cadherin antibody LS-C796922 is an unconjugated mouse monoclonal antibody to human E Cadherin (CDH1). Validated for Flow, IF, IHC, PMA and WB.
Target
Human CDH1 / E Cadherin
Synonyms
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
UMAB184
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
The extracellular domain of human CDH1 (NP_004351) produced in human cells.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100 - 1:200)
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • Protein Microarray
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDH1 / E Cadherin
P12830 NM_004360 NP_004351.1

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Featured Products

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Species: Mouse, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
CDH1 / E Cadherin Antibody - Anti-E Cadherin antibody IHC of human, spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
IHC‑plus™ CDH1 / E Cadherin Antibody (clone 3F4) LS‑B4297
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay
CDH1 / E Cadherin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CDH1 / E Cadherin Antibody LS‑B12414
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot
CDH1 / E Cadherin Antibody - Western blot using CDH1 mouse monoclonal antibody against LNCAP (1),A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
CDH1 / E Cadherin Antibody (clone 7H12) LS‑C169271
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
CDH1 / E Cadherin Antibody - Anti-E Cadherin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ CDH1 / E Cadherin Antibody (aa1‑50) LS‑B5995
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CDH1 / E Cadherin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/20/2025
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