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Antibodies > CDC25B > LS-C801073
 
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Catalog Number Size Price
LS-C801073-100 100 µl (1 mg/ml) $379 
LS-C801073-200 200 µl (1 mg/ml) $421 
CDC25B Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - 1:200 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CDC25B Antibody - Western blot analysis of CDC25B phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CDC25B Antibody - Western blot analysis of Phospho-CDC25B (Ser323) expression in various lysates
CDC25B Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - 1:200 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CDC25B Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CDC25B Antibody - Western blot analysis of CDC25B phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CDC25B Antibody - Western blot analysis of Phospho-CDC25B (Ser323) expression in various lysates
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Polyclonal Rabbit anti‑Human CDC25B Antibody (phospho‑Ser323, IHC, IF, WB) LS‑C801073

Polyclonal Rabbit anti‑Human CDC25B Antibody (phospho‑Ser323, IHC, IF, WB) LS‑C801073

Submit Review LSBio Guarantee
Antibody:
CDC25B Rabbit anti-Human Polyclonal (pSer323) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801073-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDC25B Rabbit anti-Human Polyclonal (pSer323) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25B antibody LS-C801073 is an unconjugated rabbit polyclonal antibody to CDC25B (pSer323) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CDC25B
Synonyms
CDC25B | CDC25B phosphatase | Cell division cycle 25B | M-phase inducer phosphatase 2 | CDC25HU2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human CDC25B around the phosphorylation site of Serine 323.
Epitope
pSer323
Specificity
Phospho-CDC25B (Ser323) Antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 323.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25B
P30305 NM_021872 NP_068658.1

Publications (0)

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Applications: IHC, IHC - Paraffin, Western blot
CDC25B Antibody - Western blot of Phospho-Cdc25B (S323) antibody
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CDC25B Antibody - CDC25B antibody AVARP03041_P050-NP_068659-CDC25B (cell division cycle 25 homolog B (S. pombe)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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See all CDC25B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025
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