Human CD99

Mouse

Human, Rat (tested or 100% immunogen sequence identity)

IgM,k Monoclonal

SPM586

Unconjugated

PEG purified

Unmodified. Also available Azide-free, BSA-free.

Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Human CD99

• IHC
• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This mAb shows a very similar reactivity to other CD99 mAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. Optimal dilution of the CD99 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14209 NCBI: NM_002414 NP_002405.1